m hominis reference strain pg21  (ATCC)

Journal: Frontiers in Microbiology

Article Title: A genome-wide investigation of Mycoplasma hominis genes associated with gynecological infections or infertility

doi: 10.3389/fmicb.2025.1561378

Figure Lengend Snippet: COG classification of genes. (A) Clusters of orthologous genes (COGs) distribution among the 52 M. hominis strains associated with infertility. (B) COGs distribution among the 10 M. hominis strains associated with genital infections. Bars in green, red, and blue, represent the core genome, the accessory genes (shell genes), and unique genes (cloud genes), respectively.

Article Snippet: The genome size of M. hominis reference strain PG21 (ATCC 23114) was estimated to be around 665 kb (Calcutt and Foecking, ), thus exceeding Mycoplasma genitalium genome (580 kb), the smallest genome of any organism that can be grown in pure culture (Fookes et al., ), by only 85 kb.

Techniques:

Journal: Frontiers in Microbiology

Article Title: A genome-wide investigation of Mycoplasma hominis genes associated with gynecological infections or infertility

doi: 10.3389/fmicb.2025.1561378

Figure Lengend Snippet: Single nucleotide polymorphism (SNP) distribution among M. hominis strains associated with gynecological infections (red boxplot) and infertility (blue boxplot). Student's t -test was used to assess for statistical significant difference ( P < 0.05) between the mean SNPs count values of strains linked to infertility and gynecological infections. * Represents the mean SNP count of each boxplot.

Article Snippet: The genome size of M. hominis reference strain PG21 (ATCC 23114) was estimated to be around 665 kb (Calcutt and Foecking, ), thus exceeding Mycoplasma genitalium genome (580 kb), the smallest genome of any organism that can be grown in pure culture (Fookes et al., ), by only 85 kb.

Techniques:

Journal: Frontiers in Microbiology

Article Title: A genome-wide investigation of Mycoplasma hominis genes associated with gynecological infections or infertility

doi: 10.3389/fmicb.2025.1561378

Figure Lengend Snippet: TreeWAS-based phylogenetic tree illustrating the relatedness between the 62 Tunisian M. hominis clinical strains based on pathotype association. Strains in red are associated with gynecological infections pathotype and strains in blue are associated with infertility pathotype.

Article Snippet: The genome size of M. hominis reference strain PG21 (ATCC 23114) was estimated to be around 665 kb (Calcutt and Foecking, ), thus exceeding Mycoplasma genitalium genome (580 kb), the smallest genome of any organism that can be grown in pure culture (Fookes et al., ), by only 85 kb.

Techniques:

Journal: BMC Genomics

Article Title: Generation of Mycoplasma hominis gene-targeted mutants by targeting-induced local lesions in genomes (TILLING)

doi: 10.1186/s12864-018-4917-1

Figure Lengend Snippet: Effects of EMS mutagenesis on M. hominis survival. Concentrations of M. hominis PG21 were determined after treatment with various concentration of EMS for 1.5 h and 3 h ( a ). Three independent experiments were performed. The percentage of killing was calculated ( b ). In accordance with the literature, a 75% killing rate corresponding to exposure to approximately 9 mg/ml EMS for 3 h was chosen to generate the M. hominis PG21 mutant library

Article Snippet: The M. hominis PG21 reference strain was from ATCC (ATCC 23114).

Techniques: Mutagenesis, Concentration Assay

Journal: BMC Genomics

Article Title: Generation of Mycoplasma hominis gene-targeted mutants by targeting-induced local lesions in genomes (TILLING)

doi: 10.1186/s12864-018-4917-1

Figure Lengend Snippet: vaa - and oppA -mutants obtained by screening the 1200 EMS-generated M. hominis clone library

Article Snippet: The M. hominis PG21 reference strain was from ATCC (ATCC 23114).

Techniques: Mutagenesis

Journal: BMC Genomics

Article Title: Generation of Mycoplasma hominis gene-targeted mutants by targeting-induced local lesions in genomes (TILLING)

doi: 10.1186/s12864-018-4917-1

Figure Lengend Snippet: Mutations harboured by EMS-generated M. hominis mutants in comparison to the sequence of the M. hominis PG21 strain not exposed to EMS

Article Snippet: The M. hominis PG21 reference strain was from ATCC (ATCC 23114).

Techniques: Comparison, Sequencing, Mutagenesis

Journal: BMC Genomics

Article Title: Generation of Mycoplasma hominis gene-targeted mutants by targeting-induced local lesions in genomes (TILLING)

doi: 10.1186/s12864-018-4917-1

Figure Lengend Snippet: Adhesion to HeLa cells of EMS-generated M. hominis vaa -mutants. M. hominis cells were added to immobilized HeLa cells in triplicate and incubated 4 h at 37 °C. After removal of unbound M. hominis cells by washing, the adherent M. hominis cells were quantified by real-time PCR. One representative experiment of three is shown

Article Snippet: The M. hominis PG21 reference strain was from ATCC (ATCC 23114).

Techniques: Generated, Incubation, Real-time Polymerase Chain Reaction

Journal: BMC Genomics

Article Title: Generation of Mycoplasma hominis gene-targeted mutants by targeting-induced local lesions in genomes (TILLING)

doi: 10.1186/s12864-018-4917-1

Figure Lengend Snippet: ecto ATPase activity of EMS-generated M. hominis oppA -mutants. ecto ATPase activity of EMS-generated Mho369 and Mho940 M. hominis mutants and of M. hominis PG21 parental strain was determined by measuring the release of free phosphate per 10 10 cells as a function of the ATP concentration. The data represent the means and standard errors of five (Mho369) and four (Mho940 and PG21) independent experiments with triplicate samples in each experiment. V max , maximum rate, K m , Michaelis-Menten constant, 95% IC, 95% confidence interval

Article Snippet: The M. hominis PG21 reference strain was from ATCC (ATCC 23114).

Techniques: Activity Assay, Generated, Concentration Assay